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You are here:Home » Molecular Biology » Growth Factors-GM-CSF » Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Mouse (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl

Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Mouse (GM-CSF, Burst
Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony
Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl

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Specifications

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a potent species specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages, and eosinophils. Murine GM-CSF is a 14.0kD protein containing 125 amino acid residues.
Catalog #G8950-20A
Biological Activity Determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells. The ED50 is  0.2ng/ml, corresponding to a specific activity of 5x10e6u/mg.
Responding Cells (partial list) Certain BM Precursor cells. For most in vitro applications, murine GM-CSF exerts its biological activity in the concentration range of 0.05-0.5ng/ml.
Amino Acid Sequence The sequence of the first five N-terminal amino acids was found to be Met-Ala-Pro-Thr-Arg.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
Molecular Weight14.2kD
SourceMurine, recombinant protein expressed in E. coli
Purity98% by SDS-PAGE and HPLC. Endotoxin; 0.1ng/ug (1EU/ug)
Concentration~0.1mg/ml
FormSupplied as a liquid in 20mM acetic acid. Dilute to working concentration with dH2O or aqueous buffers, 0.1% HSA or BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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