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You are here:Home » Molecular Biology » Growth Factors-GM-CSF » Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, GMCSF, Colony-stimulating Factor, CSF, Colony Stimulating Factor 2 (Granulocyte-macrophage), CSF2, MGC131935, MGC138897, Molgramostin, Sargramostim)

Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, GMCSF,
Colony-stimulating Factor, CSF, Colony Stimulating Factor 2 (Granulocyte-macrophage),
CSF2, MGC131935, MGC138897, Molgramostin, Sargramostim)

Pricing

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Specifications

Catalog #G8951-26T
SourceRecombinant corresponding to 127aa of human Granulocyte Macrophage-Colony Stimulating Factor expressed in E.coli is a single, non-glycosylated, polypeptide chain.
Molecular Weight~14.5kD
Biological ActivityThe ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less then 0.1ng/ml, corresponding to a Specific Activity of 11.1x10 6IU/mg.
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 0.963 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~14.5kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
ConcentrationNot determined
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesGranulocyte-macrophage Colony-stimulating Factor


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