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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent and suitable for a variety of applications.
Catalog #	G8965-01
Applications	Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:500-1:1000
	ELISA: 1:1000-1:10,000
	Immunohistochemistry: 1:1000.
	Mouse brain neural cells (1:1000) RT, overnight using secondary antibody anti-rat IgG-Cy3 (1:300) RT, 1hr. Data courtesy of Yoshihiro Yoshyihara RIKEN Brain Science Institute.
	Optimal dilutions to be determined by the researcher.
Hybridoma	Mouse GF200
Source	Ascites
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	GF200
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.02% sodium azide before the addition of glycerol to 40%.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Purified recombinant His-GFP full length fusion protein (Aequorea victoria).
Specificity	Reacts with Green Fluorescent Protein (GFP) and crossreacts with Blue Fluorescent Protein (BFP).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.