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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Green Fluorescent Protein (GFP) (FITC)

Anti -Green Fluorescent Protein (GFP) (FITC)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Purified E IH FC IF
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm)and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence.The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications.
Catalog #G8965-04E2
ApplicationsSuitable for use in ELISA, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: Tested on prokaryotic (E. coli) and eukaroytic (CHO cells) expression systems. Detected GRP containing inserts.
Immunohistochemistry: Frozen sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~1mg/ml
Form0.02M Potassium phosphate, 0.15M sodium chloride, [pH7.2]
PurityPurified
ImmunogenGreen Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish, Aequorea victoria.
SpecificityRecognizes GFP and its variants. Species Crossreactivity: Does not react with human, mouse and rat proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesGreen Fluorescent Protein


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