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Anti -Green Fluorescent Protein (GFP) (FITC)
Anti -Green Fluorescent Protein (GFP) (FITC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Purified |
E IH FC IF |
|
| GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm)and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence.The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications. | | | Catalog # | G8965-04E2 | | Applications | Suitable for use in ELISA, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: Tested on prokaryotic (E. coli) and eukaroytic (CHO cells) expression systems. Detected GRP containing inserts. | | Immunohistochemistry: Frozen sections. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Concentration | ~1mg/ml | | Form | 0.02M Potassium phosphate, 0.15M sodium chloride, [pH7.2] | | Purity | Purified | | Immunogen | Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish, Aequorea victoria. | | Specificity | Recognizes GFP and its variants. Species Crossreactivity: Does not react with human, mouse and rat proteins. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Green Fluorescent Protein |
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