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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light.
Catalog #	G8965-05
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	ELISA: 1:40,000. Sandwich or capture for detection of wild type, recombinant and enhanced forms of GFP.
	Western Blot: 1:400-1:2000; A 33kD band is detected from HeLa cells expressing recombinant GFP.
	Immunohistochemistry: 1:200-1:1000; works on frozen sections fixed with ice cold methanol.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Purified recombinant GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from Aequorea victoria.
Specificity	Recognizes Green Fluorescent Protein (GFP) and its variants. Assay by IEP resulted in a single precipitin arc against anti-goat serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against human, mouse or rat serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.