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Green fluorescent protein, aa1-238, Recombinant, Aequorea victoria (GFP)
Green fluorescent protein, aa1-238, Recombinant, Aequorea victoria (GFP)
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| GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. | | | Catalog # | G8965-05A | | Source | Recombinant GFP protein, expressed in E.coli. | | Molecular Weight | 26.8kD (238aa) | | Endotoxin Level | 1.0 EU /1ug of protein (LAL). | | AA Sequence | MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD | | HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 26.8kD | | Source | E. coli | | Purity | 95% by SDS-PAGE | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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