Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.

Western Blot: 0.5-2ug/ml detects Green Fluorescent Protein from 50ug of rat brain lysate containing 25ng of purified Green Fluorescent Protein. Also detected a GFP-fusion protein from a transfected E. coli cell lysate. Rat brain lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8965-06 (1ug/ml). Proteins were visualized using a rabbit anti-chicken secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: Reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein. Optimal dilutions to be determined by the researcher.

I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch

rat brain lysate, cells expressing Green Fluorescent Protein

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

His-tagged green fluorescent protein of Aequorea victoria.

Supplied as a liquid in 100mM Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol.

Purified by ammonium sulfate precipitation and PEG.

Recognizes Green Fluorescent Protein (GFP), Mr 30kD, and GFP-fusion proteins. Species Crossreactivity: Vertebrates