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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Green Fluorescent Protein (GFP)

Anti -Green Fluorescent Protein (GFP)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Purified B IC
Catalog #G8965-06
Green fluorescent protein (GFP) is a 29kD fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488nm), GFP strongly fluoresces (Emission max 507nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects GFP from 50ug of rat brain lysate containing 25ng of purified GFP. Also detected a GFP-fusion protein from a transfected E. coli cell lysate. Rat brain lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8965-06 (1ug/ml). Proteins were visualized using a rabbit anti-chicken secondary antibody conjugated to HRP* and a chemiluminescence detection system.
Immunocytochemistry: Reported to show positive immunostaining in cells transfected with an expression vector encoding GFP.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgY
HostChicken
Concentration~1mg/ml
FormSupplied as a liquid in 100mM Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol.
PurityPurified by ammonium sulfate precipitation
ImmunogenHis-tagged green fluorescent protein of Aequorea victoria.
SpecificityRecognizes green fluorescent protein (GFP), Mr 30kD, and GFP-fusion proteins. Species Crossreactivity: Vertebrates
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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