| Catalog # | G8965-06 |
| Green fluorescent protein (GFP) is a 29kD fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488nm), GFP strongly fluoresces (Emission max | 507nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells |
| Applications | Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 0.5-2ug/ml detects GFP from 50ug of rat brain lysate containing 25ng of purified GFP. Also detected a GFP-fusion protein from a transfected E. coli cell lysate. Rat brain lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8965-06 (1ug/ml). Proteins were visualized using a rabbit anti-chicken secondary antibody conjugated to HRP* and a chemiluminescence detection system. |
| Immunocytochemistry: Reported to show positive immunostaining in cells transfected with an expression vector encoding GFP. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906 IgY, Chicken Pab Rb x Ch |
| I1906-10 IgY, Chicken (HRP) Rb x Ch |
| I1906-12 IgY, Chicken (FITC) Rb x Ch |
| I1906-14 IgY, Chicken (AP) Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 100mM Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Purified by ammonium sulfate precipitation |
| Immunogen | His-tagged green fluorescent protein of Aequorea victoria. |
| Specificity | Recognizes green fluorescent protein (GFP), Mr 30kD, and GFP-fusion proteins. Species Crossreactivity: Vertebrates |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
