Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Assay Detection » Anti -Green Fluorescent Protein (GFP)

Anti -Green Fluorescent Protein (GFP)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light.
Catalog #G8965-07
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:80,000-1:500,000. Sandwich or capture for detection of GFP recombinant proteins and enhanced forms of GFP.
Western Blot: 1:3000-1:30,000
Immunohistochemistry:1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No1001
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
PurityPurified by Protein A affinity chrmoatography.
ImmunogenGreen Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jelly sh Aequorea victoria.
SpecificityRecognized wild type and recombinant forms of GFP. No reaction is seen against RFP. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti mouse serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links