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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IP Gel

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238aa and emits green light (emission maximum at 509nm) when excited with blue or UV light.
Catalog #	G8965-07E
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation, Dot Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10K230
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in 10mM PBS, PH 7.2, 0.1% sodium azide.
Purity	Purified.
Immunogen	GFP from the jellyfish Aequorea Victoria N-terminal peptide (KLH).
Specificity	Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.