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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Green Fluorescent Protein (GFP)

Anti -Green Fluorescent Protein (GFP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IP IC
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light.
Catalog #G8965-08
ApplicationsSuitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q2156
HostMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified
ImmunogenBacterial-expressed GFP fusion protein.
SpecificityRecognizes natural GFP. Directed against a bacterial-expressed GFP fusion protein. Reactivity to eGFP or other mutant varieties is poor to non-existent.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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