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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP IH IC IF

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.
Catalog #	G8965-10B
Applications	Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Immunocytochemistry( IF): 1:100-1:1000
	Immunohistochemistry: 1:100- 1:1500.
	Western Blot: 1:250-1:2500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1 sodium azide, before the addition of glycerol to 40%.
Purity	Purified by affinity chomatography.
Immunogen	GFP fusion protein corresponding to the full length amino acid sequence (246aa) derivied from the jellyfish Aequorea victoria.
Specificity	Recognizes GFP. Species reactivity: Reacts against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP and EGFP.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.