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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Green Fluorescent Protein (GFP)

Anti -Green Fluorescent Protein (GFP)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IC IF
The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.
Catalog #G8965-10B
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunocytochemistry( IF): 1:100-1:1000
Immunohistochemistry: 1:100- 1:1500.
Western Blot: 1:250-1:2500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1 sodium azide, before the addition of glycerol to 40%.
PurityPurified by affinity chomatography.
ImmunogenGFP fusion protein corresponding to the full length amino acid sequence (246aa) derivied from the jellyfish Aequorea victoria.
SpecificityRecognizes GFP. Species reactivity: Reacts against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP and EGFP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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