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Green Fluorescent Protein, Recombinant (GFP)
Green Fluorescent Protein, Recombinant (GFP)
Pricing
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| Full-length Green Fluorescent Protein with N-terminal HIS-tag (6X), recombinant, expressed in E. coli. | | | Catalog # | G8965-10E | | GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications. | | Accession Number | AAA27722 | | Applications | Suitable for use as a standard for protein gels and Western Blots (~28kD). Also suitable for calibration of uorometers and microinjection into cells and tissues. Other applications not tested. | | Recommended Dilutions | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~28kD | | Source | E. coli | | Purity | Purified using Ni-NTA agarose (~70%). | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 20% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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