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You are here:Home » Molecular Biology » MB-BioSep Assay Detection/Tags » Green Fluorescent Protein, Recombinant (GFP)

Green Fluorescent Protein, Recombinant (GFP)

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Specifications

Full-length Green Fluorescent Protein with N-terminal HIS-tag (6X), recombinant, expressed in E. coli.
Catalog #G8965-10E
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications.
Accession Number AAA27722
ApplicationsSuitable for use as a standard for protein gels and Western Blots (~28kD). Also suitable for calibration of uorometers and microinjection into cells and tissues. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage, store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~28kD
SourceE. coli
PurityPurified using Ni-NTA agarose (~70%).
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 20% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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