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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -GFP (Green Fluorescent Protein)

Anti -GFP (Green Fluorescent Protein)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from the Pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and the demonstration that GFP can be expressed as a functional transgene, GFP has become a unique new tool with exciting applications in cell, developmental and molecular biology. GFP is an ideal fluorescent probe: its fluorescence is not species specific (it has been expressed from bacteria, yeast, plant to mammalian cells); it can fuse with proteins of interest without interfering significantly with their assembly or function.
Catalog #G8965-12
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:5000
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenE. coli-expressed full length GFP (green fluorescent protein).
SpecificityRecognizes wild-type GFP and its variants such as EGFP and EBFP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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