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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP IH

Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from the Pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and the demonstration that GFP can be expressed as a functional transgene, GFP has become a unique new tool with exciting applications in cell, developmental and molecular biology. GFP is an ideal fluorescent probe: its fluorescence is not species specific (it has been expressed from bacteria, yeast, plant to mammalian cells); it can fuse with proteins of interest without interfering significantly with their assembly or function.
Catalog #	G8965-12
Applications	Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 1:5000
	Immunoprecipitation: 1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.05% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	E. coli-expressed full length GFP (green fluorescent protein).
Specificity	Recognizes wild-type GFP and its variants such as EGFP and EBFP.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.