| The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 246 amino acids. It emits green light (emission maximum at 509nm) when excited with blue or UV light. |
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| Catalog # | G8965-20 |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA (sandwich or capture): 1:10,000-1:50,000 |
| Western Blot: 1:1000-1:5000 |
| Immunohistochemistry: 1:500-1:2500 |
| Optimal dilutions to be determined by the researcher. |
| Source | Tissue culture supernatant |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 1003 |
| Host | Mouse |
| Concentration | ~1mg/ml (UV280) |
| Form | Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% Thimerosal, 40% glycerol. Labeled with horseradish peroxidase (HRP). |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jelly sh Aequorea victoria. |
| Specificity | Recognizes wild type and recombinant Green Fluorescent Protein. Does not recognize enhanced forms of GFP. Expected to detect GFP containing inserts by Immuno uorescence Microscopy on prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-peroxidase, and puri ed and partially puri ed GFP (Aequorea victoria) serum. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
