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You are here:Home » Antibodies » Abs to Assay Detection » Anti -GFP (Green Fluorescent Protein) (APC)

Anti -GFP (Green Fluorescent Protein) (APC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified FC
AB The Green Fluorescent Protein (GFP), originally identified and isolated from the jellyfish Aequorea victoria, is a universally used fluorescent indicator, utilized as a reporter for gene expression, a cell lineage tracer, and for measurements of protein-protein interactions. GFP and chimeric proteins fused to GFP have been expressed in virtually all types of cell and are used as non-invasive fluorescent markers in living cells and organisms. GFP is ~27kD and 238aa and emits green light when excited with blue or UV light (emission peak at 509nm and excitation peak at 395nm). Given its widespread use and potential applications, several GFP mutants have been developed, including enhanced GFP (EGFP), commonly used in mammalian cells.
Catalog #G8965-22C
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 10ul Labels 1x10e6 cells
Optimal dilutions to be determined by the researcher.
Absorption 620-650nm
Emission 660-670nm
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Freezing Labeled with Allophycocyanin (APC) conjugates will result in a substantial loss of activity. Further dilutions can be made in assay buffer. APC is light sensitive.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~0.01mg/ml
FormSupplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to aa2-238 from GFP expressed in E. coli (AAB65663).
SpecificityRecognizes GFP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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