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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Green Fluorescent Protein (GFP), X-Adsorbed (Biotin)

Anti -Green Fluorescent Protein (GFP), X-Adsorbed (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IF
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light.
Catalog #G8966
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:2000-1:10,000
ELISA: 1:10,000-1:50,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin.
PurityPurified by immunoaf nity chromatography.
ImmunogenRecombinant Green Fluorescent Protein (GFP) fusion protein corresponding to full length aa sequence (246aa) derived from the jelly sh Aequorea victoria.
SpecificityRecognizes wild type, recombinant and enhanced forms of GFP
(eGFP). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-biotin and puri ed and partially puri ed Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against human, mouse and rat serum proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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