| The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light. |
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| Catalog # | G8966 |
| Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:2000-1:10,000 |
| ELISA: 1:10,000-1:50,000 |
| Immunohistochemistry: 1:1000-1:5000 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Concentration | ~1mg/ml (UV280) |
| Form | Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin. |
| Purity | Purified by immunoaf nity chromatography. |
| Immunogen | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to full length aa sequence (246aa) derived from the jelly sh Aequorea victoria. |
| Specificity | Recognizes wild type, recombinant and enhanced forms of GFP |
| (eGFP). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-biotin and puri ed and partially puri ed Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against human, mouse and rat serum proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
