| The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light. |
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| Catalog # | G8966-05 |
| Applications | Suitable for use in ELISA, Immunoblot, Immuno uorescence and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 1:10,000-1:50,000 Sandwich or capture. Recognizes wild type, recombinant and enhanced forms of GFP. |
| Immunohistochemistry: 1:500-1:3000 |
| Immuno uorescence: Fluorochrome conjugated anti-GFP was assayed by immuno uorescence microscopy on prokaryotic |
| (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Signi cant ampli cation of signal was detected using uorochrome conjugated anti-GFP relative to the uorescence of GFP alone. |
| Western Blot: 1:1000-1:5000 Detects a 42kD band. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Goat |
| Concentration | ~1mg/ml (UV280) |
| Form | Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% Thimerosal, 40% glycerol. Labeled with horseradish peroxidase (HRP). |
| Purity | Purified by immunoaf nity chromatography. |
| Immunogen | GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jelly sh Aequorea victoria. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-peroxidase and puri ed and partially puri ed Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against human, mouse and rat serum proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
