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You are here:Home » Molecular Biology » Growth Factors-GRO » GRO alpha, Recombinant, Human (KC)

GRO alpha, Recombinant, Human (KC)

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Specifications

Three human MGSA/GRO genes encode 3 highly related chemokines, MGSA/GRO alpha, -beta and -gamma. All 3 MGSA/GRO proteins bind to the same receptors, but with differing affinities, and stimulate a number of biological responses including chemotaxis, angiogenesis, and growth regulation. Melanoma growth stimulatory activity/gro alpha (MGSA), a member of the alpha-chemokine family, is produced by a variety of dermal and epidermal cells and can act in a paracrine and autocrine fashion.
Catalog #G8975-16
DescriptionRecombinant Human GRO-alpha produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7811 Dalton. Recombinant Human GRO-alpha is purified by proprietary chromatographic techniques.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ser-Val-Ala-Thr.
Biological ActivityRecombinant Melanoma growth stimulatory activity is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human neutrophils cells using a conc. of 10.0-100.0 ng/ml.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC, using a calibrated solution of MGSA as a Reference Standard.
SolubilityIt is recommended to reconstitute the lyophilized GRO-alpha in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized MGSA/GRO-a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MGSA/GRO-alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Molecular Weight7.81kD
SourceE. coli
Purity97% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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