GRO/KC, Recombinant, Mouse (Cxcl1, Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth
Stimulating Activity, alpha), Cinc1, CINC-1, C-X-C Motif Chemokine 1, Cytokine-induced
Neutrophil Chemoattractant 1, Gro1, Growth-regulated alpha Protein, Platelet-derive
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| KC is a member of the CXC chemokine family with homology to human IL-8, a potent neutrophilic chemotactic cytokine. KC is a fundamental mediator of neutrophil recruitment in acute mast cell-dependent skin inflammation. | | | Catalog # | G8975-19M | | Description | Recombinant murine KC also nown as N51 and GRO-1 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7815 Dalton. Recombinant Murine GRO is purified by prop | | Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ile-Ala-Asn. | | Dimers and Aggregates | Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | Mouse CXCL1 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human neutrophils cells using a conc. of 10.0-100 ng/ml. | | Endotoxin | 0.1ng/ug (IEU/ug) of Mouse KC | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280 nm using the absorbency value of 0.03 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). | | 2. Analysis by RP-HPLC, using a standard solution of KC as a Reference Standard.rietary chromatographic techniques. | | Solubility | It is recommended to reconstitute the lyophilized Recombinant GRO1 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | | Stability | Lyophilized Murine N51/KC although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution GRO1 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | | Molecular Weight | 7.82kD | | Source | E. coli | | Purity | 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. | | Concentration | As reported | | Form | Supplied as a lyophilized powder without additives. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
|
|
Questions or comments about this product? Inquire here. |
|
|
Download Specifications for GRO/KC, Recombinant, Mouse (Cxcl1, Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, alpha), Cinc1, CINC-1, C-X-C Motif Chemokine 1, Cytokine-induced Neutrophil Chemoattractant 1, Gro1, Growth-regulated alpha Protein, Platelet-derive |
External Links
|
