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GRO beta, Recombinant, Human (CXCL2, MIP-2, Macrophage Inflammatory Protein 2)
GRO beta, Recombinant, Human (CXCL2, MIP-2, Macrophage Inflammatory Protein 2)
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| Recombinant Human GRO-beta is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7908 Dalton. Human GRO-beta is purified by proprietary chromatographic techniques. | | | Catalog # | G8975-23A | | Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Leu-Ala-Thr. | | Dimers and Aggregates | 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | GRO-beta is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract CXCR2 transfected 293 cells using 10.0-100.0ng/ml. | | Endotoxin | 0.1ng/ug (IEU/ug) of Human GRO-beta. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280nm. | | 2. Analysis by RP-HPLC, using a calibrated solution of Human GRO-beta as a Reference Standard. | | Reconstitution | Reconstitute the lyophilized Human GRO-beta in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | E. coli | | Purity | 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. | | Form | Supplied as a lyophilized powder. No additives. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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