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You are here:Home » Growth Factors, Cytokines » Growth Factors-GRO » GRO beta, Recombinant, Human (CXCL2, MIP-2, Macrophage Inflammatory Protein 2)

GRO beta, Recombinant, Human (CXCL2, MIP-2, Macrophage Inflammatory Protein 2)

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Specifications

Recombinant Human GRO-beta is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7908 Dalton. Human GRO-beta is purified by proprietary chromatographic techniques.
Catalog #G8975-23A
Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Leu-Ala-Thr.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityGRO-beta is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract CXCR2 transfected 293 cells using 10.0-100.0ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of Human GRO-beta.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of Human GRO-beta as a Reference Standard.
ReconstitutionReconstitute the lyophilized Human GRO-beta in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
FormSupplied as a lyophilized powder. No additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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