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You are here:Home » Growth Factors, Cytokines » Growth Factors-GDF » Growth and Differentiation Factor 5, Recombinant, Human (GDF5)

Growth and Differentiation Factor 5, Recombinant, Human (GDF5)

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Specifications

Recombinant human GDF-5 produced in E. coli is a homodimer comprised of a non-glycosylated polypeptide chain containing 2 x 117 amino acids and having a total molecular mass of 26.8kD. To enable bacterial expression of rhGDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lys. GDF5 is purified by proprietary chromatographic techniques.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Arg-Gln-Gly.
Catalog #G8989-45
Biological ActivityHuman GDF-5 is fully biologically active when compared to standard. Induction of alkaline phosphatase acrtivity in ATDC5 cells: EC50 = 40Nm.
EndotoxinLess than 0.1ng/ug (IEU/ug) of GDF-5.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of GDF-5 as a Reference Standard.
Storage and StabilityLyophilized powder is stable for at least 12 months at -20°C Reconstituted product must be stored in aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight26.8kD
SourceE. coli
Purity95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug).
FormSupplied as a lyophilized powder without any additives. Reconstitute with sterile 20mM AcOH to 100ug/ml, which can then be further diluted to other aqueous solutions with 0.1% BSA or HSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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