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You are here:Home » Molecular Biology » Growth Factors-GDF » Growth and Differentiation Factor 5, Recombinant, Human (GDF-5)

Growth and Differentiation Factor 5, Recombinant, Human (GDF-5)

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Specifications

GDF-5 acts as an inhibitor of nerve tissue growth. It is produced as a homodimer, nonglycosylated polypeptide chain containing 2X117aa with a molecular weight of 26.8kD. To enable bacterial expression of rhGDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lys.
Catalog #G8989-45D
SourceRecombinant corresponding to human GDF-5 expressed in E. coli.
Biological ActivityThe biological activity of the protein was measured by induction of alkaline phosphatase activity in ATDC5 cells. The EC50 is 30-50nM.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 20mM HOAc. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~26.8kD
SourceE.coli
Purity~95% (SDS-PAGE and HPLC). Endotoxin: <0.1ng/ug.
ConcentrationAs reported
FormSupplied as a lyophilized powder. No additives added. Reconstitute in 20mM HOAc to 0.1-1.0mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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