Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Hormones, Steroids » Anti -Growth Hormone, Human (hGH)

Anti -Growth Hormone, Human (hGH)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Catalog #G9000-07
hGH is produced by the somatotroph cells in the pituitary. hGH acts through binding to membrane receptors that belong to the cytokine receptor superfamily. Ligand binding induces receptor dimerization and activation of the receptorassociated kinase JAK2; this results in phosphorylation of the kinase itself, of the receptor, and of many cellular proteins including the Stat proteins as well as adaptors leading to the activation of the Ras/MAP kinase pathway and of the PI-3 kinase pathway.
ApplicationsSuitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1:2000-4000 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilution to be determined by researcher.
Positive ControlRecombinant human growth hormone. Human anterior pituitary
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No3F199
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See G9000-07X.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified rcombinant human growth hormone.
Cellular LocalizationCytoplasmic MW of Antigen: ~22kD.
SpecificityRecognizes human Growth Hormone (hGH).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links