| The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell. |
|
| Catalog # | G9005 |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA (Peptide): 1:1000 |
| Optimal dilutions to be determined by researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa385-394 RMHLRQYELL at the C-terminal region of Gs alpha, conjugated to KLH (NM_000516). Species Sequence Homology: human |
| Specificity | Recognizes both the large (MW ~52kD) and small (MW ~45kD) forms of Gs alpha. Does not crossreact with other G proteins. Species Crossreactivity: human and mouse |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | GSA, GSP, POH, GPSA, NESP, GNAS1, GNASXL, NESP55, G-s-alpha neuroendocrine secretory protein 55, guanine nucleotide regulatory protein, paternally expressed G protein XL alpha S, adenylate cyclasestimulating G alpha protein, guanine nucleotide binding pro |
