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You are here:Home » Molecular Biology » MB-Enzymes, Methyltransferase » Guanidinoacetate N-methyltransferase isoform, Recombinant Human, aa1-256, His-Tag (GAMT)

Guanidinoacetate N-methyltransferase isoform, Recombinant Human, aa1-256, His-Tag (GAMT)

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Specifications

Guanidinoacetate N-methyltransferase isoform a, also known as GAMT is a methyltansferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl domor. This enzyme participates in the two-step synthesis of the compound creatine from the protein building blocks glycine, arginine, and methionine. It is involved in providing energy for muscle contraction, and is also important in nervous system functioning. Also GAMT is active in the liver, pancreas, and kidneys. Recombinant human GAMT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Catalog #G9028
SourceRecombinant corresponding to human GAMT, his-tagged, expressed in E. coli.
AA sequenceMGSSHHHHHH SSGLVPRGSH MSAPSATPIF APGENCSPAW GAAPAAYDAA DTHLRILGKP VMERWETPYM HALAAAASSK GGRVLEVGFG
MAIAASKVQE APIDEHWIIE CNDGVFQRLR DWAPRQTHKV IPLKGLWEDV APTLPDGHFD GILYDTYPLS EETWHTHQFN FIKNHAFRLL
KPGGVLTYCN LTSWGELMKS KYSDITIMFE ETQVPALLEA GFRRENIRTE VMALVPPADC RYYAFPQMIT PLVTKG
Storage and StabilityAliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight28.4 kDa
Purity~95% (SDS-PAGE) Purified by immunoaffinity chromatography.
Concentration~1mg/ml
FormSupplied as a liquid in 20mM Tris-HCl, pH8.0, 1mM DTT, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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