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You are here:Home » Antibodies » Abs to G Proteins » Anti -GNAS (Guanine Nucleotide Binding Protein G(s) Subunit alpha Isoforms XLas, GNAS1, GPSA, GSA, Adenylate Cyclase Stimulating G alpha Protein, AHO, C20orf45, Extra Large alphas Protein, XLalphas, GSP, MGC33735, NESP, PHP1A, PHP1B, PHP1C, POH)

Anti -GNAS (Guanine Nucleotide Binding Protein G(s) Subunit alpha Isoforms XLas, GNAS1,
GPSA, GSA, Adenylate Cyclase Stimulating G alpha Protein, AHO, C20orf45, Extra Large
alphas Protein, XLalphas, GSP, MGC33735, NESP, PHP1A, PHP1B, PHP1C, POH)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
The GNAS1 gene encodes the alpha subunit of the G protein Gs, which couples receptor binding by several hormones to activation of adenylate cyclase (Hayward et al., 1998). This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript exists, and this antisense transcript and one of the transcripts are paternally expressed, produce noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
Catalog #G9039-42A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:3000
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7, 0.01% thimerosal, 10% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa164-394 from human GNAS.
SpecificityRecognizes human GNAS. Species sequence homology: mouse (88%), rat (100%), bovine (100%) and porcine (90%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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