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You are here:Home » Antibodies » Abs to GTPase, Rab, Ras, Rho Proteins » Anti -Guanine Neculeotide Exchange Factor H1 (GEF-H1, Rho/Rac guanine nucleotide exchange factor 2, Proliferating cell nucleolar antigen p40, Lfc)

Anti -Guanine Neculeotide Exchange Factor H1 (GEF-H1, Rho/Rac guanine nucleotide exchange
factor 2, Proliferating cell nucleolar antigen p40, Lfc)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IF
GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments. Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin.
Catalog #G9039-54
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:50
ELISA: 1:50
Immunoprecipitation: 1:50
Immunohistochemistry: frozen
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone NoB4/7
HostMouse
SourceCanine
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1mg/m l0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRaised against a Guanine nucleotide exhange factor H1 protein fraction isolated from detergent extracts of MDCK cells.
SpecificityRecognizes canine guanine nucleotide exchange factor H1 (GEF-H1). Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesRho/Rac Guanine Nucleotide Exchange Factor 2, ARHGEF2, GEF, GEFH1


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