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Abs to GTPase, Rab, Ras, Rho Proteins
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Anti -Guanine Neculeotide Exchange Factor H1 (GEF-H1, Rho/Rac guanine nucleotide exchange factor 2, Proliferating cell nucleolar antigen p40, Lfc)
Anti -Guanine Neculeotide Exchange Factor H1 (GEF-H1, Rho/Rac guanine nucleotide exchange
factor 2, Proliferating cell nucleolar antigen p40, Lfc)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP IH IF |
|
| GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments. Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin. | | | Catalog # | G9039-54 | | Applications | Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:50 | | ELISA: 1:50 | | Immunoprecipitation: 1:50 | | Immunohistochemistry: frozen | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | B4/7 | | Host | Mouse | | Source | Canine | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 1mg/m l0.1% BSA, 0.02% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Raised against a Guanine nucleotide exhange factor H1 protein fraction isolated from detergent extracts of MDCK cells. | | Specificity | Recognizes canine guanine nucleotide exchange factor H1 (GEF-H1). Species Crossreactivity: human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Rho/Rac Guanine Nucleotide Exchange Factor 2, ARHGEF2, GEF, GEFH1 |
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