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You are here:Home » Antibodies » Antibodies-MHC » Anti -HA-1 (HLA-HA1, HMHA1, KIAA0223, Minor Histocompatibility Antigen HA-1, mHag HA-1, Minor Histocompatibility Protein HA-1) (Azide free) (HRP)

Anti -HA-1 (HLA-HA1, HMHA1, KIAA0223, Minor Histocompatibility Antigen HA-1, mHag HA-1,
Minor Histocompatibility Protein HA-1) (Azide free) (HRP)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
The minor histocompatibility antigen (mHags), HA-1 is a immunogenic alloantigen shown to be responsible for graft-versus-host disease (GVHD) in HLA-identical bone marrow transplantation. The antigen has two known alleles resulting in a single amino acid polymorphism. The HA-1H allele encodes histidine, whereas the HA-1R allele encodes arginine.
Catalog #H1000-50B-HRP
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityStore product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note Applications are based on unconjugated antibody.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenKLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa883-912 from the center region of human HA-1.
SpecificityRecognizes human HA-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesHistocompatibility (Minor) HA-1

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