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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH IF

Hakai is a c-Cbl-like protein. Hakai is an E-cadherin binding protein, which has been identified as an E3 ubiquitin-ligase. Hakai contains SH2, RING, zinc-finger and proline-rich domains. Hakai interacts with E-cadherin in a tyrosine phosphorylation-dependent manner, inducing ubiquitination of the E-cadherin complex. Expression of Hakai in epithelial cells disrupts cell-cell contacts and enhances endocytosis of E-cadherin and cell motility. By promoting the endocytosis and dynamic recycling or destruction of E-cadherin complexes, Hakai is able to control epithelial-mesenchymal transitions under physiological and pathological conditions.
Catalog #	H1741
Applications	Suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended Dilutions
	Immunohistochemistry: 1:50
	Immunofluorescence: 1:50
	Western Blot: 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity	Purified by Protein A affininty chromatography.
Immunogen	Human Hakai
Specificity	Recognizes human Hakai.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.