| This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. |
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| Catalog # | H1779-02D |
| Applications | Suitable for use in ELISA and Western Blot. Other applications have not been tested. |
| Recommended Dilutions | |
| Peptide ELISA Titer: 1:128,000 |
| Western Blot: 0.1-0.3ug/ml detects ~150kD band observed in Human Brain lysates (calculated MW of 130kD according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9(12):1721-7; PMID: 10915759). In transfected HEK293 transiently expressing Human TSC1 a band of approx. 120kD is observed. This band is not observed in the non-transfected HEK293. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide. |
| Purity | Purified by peptide affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to C-QLHIMDYNETHHEH, from the C Terminus of human Hamartin (NP_000359.1). |
| Specificity | Recognizes human Hamartin. This antibody is expected to recognise tuberous sclerosis isoform 1 (NP_000359.1) only. Species sequence homology: mouse, rat, canine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | tumor suppressor, tuberous sclerosis 1 protein, TSC, MGC86987, LAM, KIAA0243, HGNC:12362, tuberous sclerosis 1, hamartin, TSC1 |
