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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IH

Hairy cell leukemia (HCL) is a chronic B-cell lymphoproliferative disorder characterized by pancytopenia and variable infiltration of the reticuloendothelial system with hairy lymphocytes. The lymphocytes present characteristic citoplasmatic projections and are positive for tartrate-resistant acid phosphatase (TRAP). It is more common in men than women and has a median age of diagnosis of 52yr. It is typically seen in middle-aged men, characterized by pancytopenia, splenomegaly, immunologic abnormalities, and morphologically typical neoplastic mononuclear cells in the blood, bone marrow, liver, spleen, and other tissues. In addition to pyogenic infections, patients are susceptible to unusual organisms including atypical mycobacterium, legionella, and fungi. Diagnosis for HCL is confirmed by demonstration of hairy cells in biopsy specimens from the bone marrow or spleen or in peripheral blood. Splenectomy is the standard initial treatment of choice. Recently, the nucleosides pentostatin (2'-deoxycoformycin) (DCF) and 2'-chlorodeoxyadenosine (2-CdA) have been shown to produce greater numbers of durable complete remissions with curative potential in patients with HCL.
Catalog #	H1826-90
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry (FFPE): 1:15-1:30. Incubate for 30 minutes at RT. Requires high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining.
	Immunohistochemistry: Frozen sections
	Optimal dilutions to be determined by the researcher.
Positive Control	Tonsil
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	11E213
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid, 0.05% sodium azide.
Purity	Supernatant
Immunogen	Deau cell line established from a large cell lymphoma. Cellular Localization: Cell membrane, cytoplasmic.
Specificity	Recognizes human Hairy Cell Leukemia. Reacts with a subset of B-lymphocytes localized in the follicular mantle zone. It reacts with 97% of hairy cell leukemia cases. Shows strong positive staining of about 35% of cases of high grade B cell lymphomas.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.