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You are here:Home » Antibodies » Antibodies-Ion Channel » Anti -HCN1 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 1)

Anti -HCN1 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 1)


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Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Biological rhythms (beating of the heart, circadian sleep cycles, respiration, and the release of hormones) are necessary to sustain life. Heart beating is the most reliable and rhythmic biological phenomenon. Cardiac pacemaking is produced by the slow diastolic depolarization phase of the action potential. The hyperpolarization-activated cation current (termed If, Ih, or Iq) plays a key role in the in the initiation and modulation of cardiac and neuronal pacemaker depolarization. The generation of cardiac pacemaker potentials relies on a complex interplay between at least four different types of cation channels: T- and L-type Ca2+ channels, K+ channels, and a cation channel termed If (synonymous names are Ih and Iq). The If channel has been designated as "pacemaker" channel because it reveals unique features that are believed to be a prerequisite for pacemaker activity. Recently, the hyperpolarization-activated cyclic nucleotide-gated families of ion channel proteins (HCN1-4) have been identified as the "pacemaker" channel. The amino acid sequences of HCNs predict a structure similar to that of voltage-gated (Kv) channels and cyclic nucleotide-gated (CNG) channels. HCNs proteins (HCN1-4) are characterized by six transmembrane domains (S1-S6), including a including a positively charged voltage-sensing S4 segment and an ion-conducting pore between S5 and S6. In the C terminus the HCNs carry a cyclic nucleotide-binding domain (CNBD), a motif found in several cyclic nucleotide-binding proteins. The core region of HCNs channels (S1 to the C terminus of the CNBD) is highly conserved, whereas the cytoplasmic N and C-termini, vary considerably in their length and share only weak sequence homology. The functional HCN, like other members of the superfamily of voltage-gated cation channels, channels probably assemble into tetrameric complexes.
Catalog #H1827-25D
HCN1, also known as Brain cyclic nucleotide channel gated 1, BCNG1 or HAC-2 (mouse/rat 910 aa, human 749-aa), is primarily expressed in brain. In mouse, HCN1 is mainly expressed in the neocortex, the CA1 region of the hippocampus, the superior colliculus, and in the molecular cell layer of the cerebellum.
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
RecommendWestern Blot 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA 1:10,000-1:100,000 using 50-100ng of control peptide/well.ed Dilution:.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
ImmunogenA 14-aa peptide sequence, mapping near the cytoplasmic, N-terminus of human/mouse HCN1 (1) was synthesized, coupled to KLH
SpecificityThe peptide is 100% conserved in human, mouse and 92% in rabbit and rat HCN1. No significant sequence homology is seen with other HCNs or any other protein. Antibody reactivity in various species is not known. The control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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