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You are here:Home » Antibodies » Abs to Ion Channel » Anti -HCN4 (Hyperpolarization-activated Cyclic Nucleotide-gated Potassium Channel 4)

Anti -HCN4 (Hyperpolarization-activated Cyclic Nucleotide-gated Potassium Channel 4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli.
Catalog #H1827-28
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:200 using ECL on rat brain membranes.
Immunohistochemistry: mouse brain sections
Note: Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal dilutions to be determined by the researcher.
Control Antigen IncludedH1827-28A: HCN4, Control Antigen
Reconstitute with 100ul sterile PBS. For Positive Control in Western Blot, use 20ng of protein per Minigel lane. For negative control preincubate 3ug of fusion protein with 1ug antibody for 1hour at RT.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.05% sodium azide. Reconstitute with 50ul sterile ddH2O.
PurityPurified by affinity chromatography.
ImmunogenGST fusion protein corresponding to aa119-155 (HGHLH DSAEE RRLIA EGDAS PGEDR TPPGL AAEPE RP) of human HCN4
(Accession XP_007730, NP_005468, CAB42604, CAB52754)
SpecificityRecognizes HCN4. Species Crossreactivity: mouse, rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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