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You are here:Home » Antibodies » Abs to Ion Channel » Anti -HCN4 (Hyperpolarization-activated Cyclic Nucleotide-gated Potassium Channel 4)

Anti -HCN4 (Hyperpolarization-activated Cyclic Nucleotide-gated Potassium Channel 4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels. Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain.
Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Catalog #H1827-28B
Recommended DilutionsWestern Blot: 1ug/ml if results are poor, use lysate without boiling, heat at 37ºC for 15 minutes
Immunohistochemistry (Peroxidase): 0.1-1.0ug/ml
Immunocytochemistry (Peroxidase): 0.1-1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight130kD
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F710
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.4, 0.09% sodium azide, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenFusion protein corresponding to aa1019-1108 (cytoplasmic C-terminus) of rat HCN4 (Accession #Q9JKA7).
SpecificityRecognizes rat HCN4 at ~130kD. Species Crossreactivity: human and mouse. Does not cross react with other HCNs.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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