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You are here:Home » Antibodies » Abs to Adapter Proteins » Anti -HCST (DAP10, Hematopoietic Cell Signal Transducer, DNAX-activation Protein 10, Membrane Protein DAP10, Transmembrane Adapter Protein KAP10, KAP10, PIK3AP, UNQ587/PRO1157)

Anti -HCST (DAP10, Hematopoietic Cell Signal Transducer, DNAX-activation Protein 10,
Membrane Protein DAP10, Transmembrane Adapter Protein KAP10, KAP10, PIK3AP,
UNQ587/PRO1157)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Transmembrane adapter protein which associates with NKG2D to form an activation receptor NKG2D-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with NKG2D-DAP10 triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full NKG2D-HCST-mediated activation and ultimate killing of target cells. In NK cells, NKG2D-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. NKG2D-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells; indeed, melanoma cells that do not express NKG2D ligands escape from immune surveillance mediated by NK cells.
Catalog #H1827-45B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:128,000
Western Blot: 0.2-0.6ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-RSPAQEDGKVYIN, from human HCST, at C-terminal (NP_055081.1, NP_001007470.1).
SpecificityRecognizes human HCST. Species sequence homology: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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