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You are here:Home » Antibodies » Abs to Enzymes, Deacetylase » Anti -HDAC1 (Histone Deacetylase 1)

Anti -HDAC1 (Histone Deacetylase 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Catalog #H1827-51G
ApplicationsSuitable for use in ELISA, Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-1ug/ml detects HDAC1 in RIPA lysates from 3T3/A31 cells.
Immunoprecipitation: 4ug
Immunocytochemistry: Positively stains HDAC1 in human placenta and mouse embryo cells permeabilized with 0.05% Tween and fixed with 4% paraformaldehyde.
Chromatin Immunoprecipitation: Reported to immunoprecipitate enzymatically active HDAC1, and co-immunoprecipitate RbAp48 and Sp1. 4-5ug immunoprecipitates transcriptionally active chromatin containing HDAC1 from actinomycin D- treated U2OS human osteosarcoma cells.
Optimal dilutions to be determined by the researcher.
Positive ControlF4209-3T3/A31 Cell Lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.T.12A
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 50mM sodium phosphate, pH 8.0, 0.035% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide (C- EEKPEAKGVKEEVKLA) corresponding to amino acids 467-482 of mouse HDAC1. The immunizing sequence is identical in human.
SpecificityRecognizes mouse HDAC1, Mr 60kD. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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