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You are here:Home » Antibodies » Abs to Enzymes, Deacetylase » Anti -HDAC7 (Histone Deacetylase 7, DKFZP586J0917, HD7a)

Anti -HDAC7 (Histone Deacetylase 7, DKFZP586J0917, HD7a)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.
Catalog #H1827-56D
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-4ug/ml
Immunohistochemistry: 20-40ug/ml
Immunoprecipitation: 20-40ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
PurityPurified by Immunoaffinity chromatography.
ImmunogenSynthetic peptide surrounding amino acid 921 of murine HDAC7 (ID# BV-79)
SpecificityRecognizes mouse HDAC7 at ~105kD. Species Crossreactivity: human and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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