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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP IF

HDAC family members have been divided into two classes. Class I of the HDACs comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDACs comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns.
Catalog #	H1827-56L1
Applications	Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended Dilution	Western Blot: 0.5-4ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Purity	Purified by Immunoaffinity chromatography.
Immunogen	Synthetic peptide surrounding amino acid 25 of human HDAC-9 (ID# BV-71)
Specificity	Recognizes human HDAC9 at ~110-140kD. A 75kD and a 60kD bands can also be detected in some tissue lysates.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.