| HE2 is a human epididymis specific sperm binding secretory protein expressed by SPAG11 gene (chromosome 8p23-p22), a 103aa protein adopts a typical antigenic pattern with a subacrosomal equatorial distribution on the sperm head, this localization of HE2 with Beta-defensins in the epithelial defense system of epididymal duct have developed mechanism to fight ascending infections, including local production of these antimicrobial peptides and proteins. |
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| The regionalized epididymal expression of the HE2 and its isoforms suggests that they exert their effects at different times in the controlled sequence of sperm modification, maturation, binding and a direct role in fertilization. |
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| HE2 represents 3 major isoforms, HE2-alpha, HE2-beta and HE2-gamma, all variants have a common 71 residue N terminus but various C termini, these variations at C termini is due to frame shifting deletions. Thus, identical sequences and deletion, start and stop points indicate that HE2 isoforms are derived from alternative splicing of 8 or more exons of a single gene and working towards common microbial defense and sperm maturation to acquire fertilizing ability. The human HE2 protein and its isoforms are 28% conserved |
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| A gene product of rat epididymal duct, BIN1B, has been suggested to represent the rat counterpart of human HE2 protein. As HE2, BIN1B also shows structural characteristics of beta-defensins. |
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| Catalog # | H1827-97H |
| Source | 14aa peptide from Human HE2. The human HE2 control peptide is 100% conserved in all a, b, and g isoforms, with no significant homology detected in other species. |
| Location | Mid-region, Intracellular domain |
| Applications | Suitable for use in ELISA. Other applications not tested. |
| Recommended Dilution | ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (See H1827-97G). |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Purity | Purified |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder from PBS, 0.05% sodium azide. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
