Heat Shock Proteins

Kit Type

Antibodies only

Tests

840

Detection Method

Colorimetric/Fluorescent

EU Commodity Code

38220000

Shipping Temp

Blue Ice

Storage Temp

-20°C

Heat Shock Proteins (Chaperone, Cpn) Antibody Panel provides an economical means to investigate protein folding within the cell. The kit contains enough primary and secondary antibodies to perform two Western mini-blot experiments with each antibody.

Kit Components

Vol. MW Isotype H1830-40B1: HSP60 20ul 60kD Rabbit Mab IgG H1830-40B2: HSP70 20ul 72, 73kD Rabbit Mab IgG H1830-40B3: HSP40) 20ul 40kD Rabbit Mab IgG H1830-40B4: HSP90 20ul 90kD Rabbit Mab IgG H1830-40B5: Calnexin 20ul 90kD Rabbit Mab IgG H1830-40B6: PDI 20ul 57kD Rabbit Mab IgG H1830-40B7: HSF1 20ul 82kD Rabbit Mab IgG H1830-40B8: BiP 20ul 78kD Rabbit Mab IgG H1830-40B9: IgG (HRP) 100ul Goat

All antibodies are supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol

Applications

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions

Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.

Storage and Stability

Store components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

References

1. Nollen, E.A. and Morimoto, R.I. (2002) J. Cell Sci. 115, 2809-2816. 2. Fan, C.Y. et al. (2003) Cell Stress Chaperones 8, 309-316. 3. Jindal, S. et al. (1989) Mol Cell Biol 9, 2279-83. 4. Itoh, H. et al. (2002) Eur. J. Biochem. 269, 5931-5938.5. Gupta, S. and Knowlton, A.A. J. Cell Mol. Med. 9, 51-58.6. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11, 116-128.7. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292, E292-E297.8. Bergeron, J.J. et al. (1994) Trends Biochem. Sci. 19, 124-128.9. Williams, D.B. (2006) J. Cell Sci. 119, 615-623.10 Kohno, K. et al. (1993) Mol. Cell. Biol. 13, 877-890.11. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep. 6, 28-32.12. Morimoto, R.I. (1998) Genes Dev. 12, 3788-3796.13. Satyal, S.H. et al. (1998) Genes Dev. 12, 1962-1974.

USBio References

No references available

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H1830-40B Heat Shock Proteins (Chaperone, Cpn) Antibody Panel, BioAssay™

Kit Type

Tests

Detection Method

EU Commodity Code

Shipping Temp

Storage Temp

References

USBio References

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