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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Factor 1 (Heat Shock Transcription Factor 1, HSF1, HSTF1)

Anti -Heat Shock Factor 1 (Heat Shock Transcription Factor 1, HSF1, HSTF1)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified E B IP IC
HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary
for suppression of trimerization. There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form. Various experiments have suggested that HFS2 may have roles in differentiation and development.
Catalog #H1830-44K
ApplicationsSuitable for use in ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry and Gel Mobility Shift Assay. Other applications not tested.
Recommended DilutionsWestern Blot: 20ug of HeLa lysates.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10F711
HostRat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 50% glycerol.
PurityPurified
ImmunogenPurified recombinant HSF1 protein.
SpecificityRecognizes human HSF1 protein. Species Crossreactivity: mouse, rat, bovine, guinea pig, hamster, monkey, and rabbit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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