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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 20 (HSP20)

Anti -Heat Shock Protein 20 (HSP20)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IF
Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and alphaB-crystallin and may form different hetercomplexes with these proteins. The specific physiologic function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. Hsp20 can be phosphorylated in vitro by cAMP-dependant protein kinase (PKA) and cGMP-dependant protein kinase (PKG). Phosphorylated Hsp20 is proposed to interact with monomeric actin and alpha-actinin whereas dephosphorylated Hsp20 binds polymeric actin filaments. Phosphorylated Hsp20 is also associated with vasorelaxation. Dissociated Hsp20, but not aggregated Hsp20, suppresses platelet aggregation, and human platelets show specific binding sites for Hsp20. Elevated levels of dissociated Hsp20 have also been found in the plasma of cardiomyopathic hamsters. Hsp20, which immediately responds to pathological events, may act extracellularly as a regulator of platelet functions.
Catalog #H1830-48
ApplicationsSuitable for use in ELISA, Western Blot and Immunofluroescence. Other applications have not been tested.
Recommended DilutionsImmunofluorescence: Cell culture staining
Optimal dilutions to be determined by the researcher.
HybridomaSp 2/0 myeloma cells with spleen cells from Balb/c mice.
Source Ascites
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2B38
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography. (>90%)
ImmunogenRecombinant protein corresponding to human HSP20
SpecificityRecognizes human recombinant HSP20 protein and HSP20 from human and rat cardiac tissue extracts.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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