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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 25,27 (HSP25,27)

Anti -Heat Shock Protein 25,27 (HSP25,27)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IF
Catalog #H1830-49
ApplicationsSuitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and affinity purification. Other applications have not been tested.
SourceAscites
Recommended DilutionImmunofluorescence: Staining of cell cultures
Optimal dilutions to be determined by the researcher.
HybridomaHybridization of Sp 2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2B39
HostMouse
SourceChicken
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.4 with 0.1% sodium azide.
Purity>90%. Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.
ImmunogenRecombinant chicken HSP25
SpecificityCrossreaction with HSP25,27 from fish, chicken, rat, mouse, feline, canine, goat, porcine, bovine and human. The amino acid sequence homology between HSP 25 (mouse) and HSP 27 (human) is > 80%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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