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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 27 (HSP27)

Anti -Heat Shock Protein 27 (HSP27)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC
Heat shock protein (HSP) 27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells. It belongs to the small heat shock protein (HSP20) family. Within minutes of heat shock, HSP27 is phosphorylated, and relocalizes from the cytoplasm to within or around the nucleus.
Catalog #H1830-50
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detected HSP27 in 20ug of cell lysates of heat shocked human HeLa cells.
Immunocytochemistry: 10ug/ml detected nuclear staining in heat shocked HeLa cells fixed in 4% paraformaldehyde/ 2% acetic acid.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-40D: IgG, H&L (FITC) Pab Gt xRb
I1904-40M: IgG, Fc, X-Adsorbed (FITC) Pab Gt xRb
I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb
I1904-41B: IgG, F(ab')2 (FITC) Pab Gt xRb
I1904-41G: IgG, F(ab')2, X-Adsorbed (FITC) Pab Gt xRb
I1904-46N: IgG, H&L (FITC) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant human HSP27.
SpecificitySpecific for human HSP27 (27kD). Species crossreactivity: Does not crossreact with rodent and calf HSP27.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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