| Heat shock protein (HSP) 27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells. It belongs to the small heat shock protein (HSP20) family. Within minutes of heat shock, HSP27 is phosphorylated, and relocalizes from the cytoplasm to within or around the nucleus. |
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| Catalog # | H1830-50 |
| Applications | Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 0.5-2ug/ml detected HSP27 in 20ug of cell lysates of heat shocked human HeLa cells. |
| Immunocytochemistry: 10ug/ml detected nuclear staining in heat shocked HeLa cells fixed in 4% paraformaldehyde/ 2% acetic acid. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-40D: IgG, H&L (FITC) Pab Gt xRb |
| I1904-40M: IgG, Fc, X-Adsorbed (FITC) Pab Gt xRb |
| I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb |
| I1904-41B: IgG, F(ab')2 (FITC) Pab Gt xRb |
| I1904-41G: IgG, F(ab')2, X-Adsorbed (FITC) Pab Gt xRb |
| I1904-46N: IgG, H&L (FITC) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 40%. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Recombinant human HSP27. |
| Specificity | Specific for human HSP27 (27kD). Species crossreactivity: Does not crossreact with rodent and calf HSP27. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
