| Heat shock protein (HSP) 27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells. It belongs to the small heat shock protein (HSP 20) family. Within minutes of heat shock, HSP27 is phosphorylated, and relocalizes from the cytoplasm to within or around the nucleus[1,2]. |
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| Catalog # | H1830-50A |
| Applications | Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:500 detected HSP27 in 20ug of L6 cell lysates untreated and treated with anisomycin (10ug for 30 min). |
| Immunocytochemistry: 10ug/ml detected nuclear staining in heat shocked CHO cells fixed in 50% ethanol/50% acetic acid. |
| Optimal dilution determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| I1904-40D: IgG, H&L (FITC) Pab Gt xRb |
| I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb |
| I1904-46N: IgG, H&L (FITC) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Hamster |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide (AGKSEQSGAK) corresponding to C-terminal hamster HSP 27. |
| Specificity | Recognizes rodent HSP 27 (27kD). No crossreactivity was observed with human HSP 27. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
