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Anti -Heat Shock Protein 27, phosphorylated (Ser15) (HSP27)
Anti -Heat Shock Protein 27, phosphorylated (Ser15) (HSP27)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Catalog # | H1830-50F | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-2ug/ml of this lot detected phosphorylated HSP27 (Ser15) in MCF7 cells that were heat shocked for 30 minutes at 42°C, cultured for an additional 16 hours at 37°C, and then stimulated with 200uM arsenite for 30 minutes. | | Protein Phosphorylation/Immunoblot Assay | Recombinant HSP27 was phosphorylated by active MAPKAP Kinase 2 in a non-radioactive protein phosphorylation reaction. The phosphorylated HSP27 was detected by Immunoblot Analysis using 0.5-4ug/ml H1830-50F. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 200ul of 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | KLH-conjugated, synthetic peptide corresponding to aa10-20 (LLRGP[pS]WDPFR-C) of human HSP27, with a C-terminal cysteine added for conjugation purposes. The immunizing sequence has 10/11 identical amino acids in canine (Canis familiaris). | | Specificity | Recognizes phospho-HSP27 (Ser15), Mr 27kD. Does not recognize the non-phosphorylated form of HSP27. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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