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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 27, phosphorylated (Ser15) (HSP27)

Anti -Heat Shock Protein 27, phosphorylated (Ser15) (HSP27)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #H1830-50F
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml of this lot detected phosphorylated HSP27 (Ser15) in MCF7 cells that were heat shocked for 30 minutes at 42°C, cultured for an additional 16 hours at 37°C, and then stimulated with 200uM arsenite for 30 minutes.
Protein Phosphorylation/Immunoblot Assay Recombinant HSP27 was phosphorylated by active MAPKAP Kinase 2 in a non-radioactive protein phosphorylation reaction. The phosphorylated HSP27 was detected by Immunoblot Analysis using 0.5-4ug/ml H1830-50F.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 200ul of 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to aa10-20 (LLRGP[pS]WDPFR-C) of human HSP27, with a C-terminal cysteine added for conjugation purposes. The immunizing sequence has 10/11 identical amino acids in canine (Canis familiaris).
SpecificityRecognizes phospho-HSP27 (Ser15), Mr 27kD. Does not recognize the non-phosphorylated form of HSP27. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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