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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 27, phosphorylated (Ser78) (HSP27)

Anti -Heat Shock Protein 27, phosphorylated (Ser78) (HSP27)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).
Catalog #H1830-50H
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:2,000
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:250-500
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k198
HostRabbit
SourceHuman
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Serine 78 of human HSP27
SpecificityRecognizes human HSP27 when phosphorylated at Ser78.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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