Anti -Heat Shock Protein 27, phosphorylated (Ser15) (HSP27, Heat Shock Protein beta-1,
HspB1, Heat Shock 27kD Protein, Stress-responsive Protein 27, SRP27, Estrogen-regulated
24kD Protein, 28kD Heat Shock Protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH |
|
| Heat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4). | | | Catalog # | H1830-50W | | Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:2000-1:5000 | | Immunoprecipitation: 1:20 | | Immunohistochemistry: 1:100-1:250 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9H357 | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. | | Purity | Supernatant. | | Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Serine 15 of human HSP27. | | Specificity | Recognizes human Src phosphorylation on Serine 15. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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