| Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in different cell types and tissues (this protein has also been referred to as the |
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| Estrogen-Regulated 24 kD protein, hsp25 and hsp28). Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and post-translational level. In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. Human HSP27 shares greater than 50% homology with the low molecular weight Drosophila HSP and mammalian crystalline lens protein. Because of the estrogen responsive nature of HSP27, the protein has been extensively studied in human estrogen responsive tissue such as cervix, endometrium and breast tissue. Recently, Hsp27 has been implicated as a regulator of breast tumor blood vessel growth, as an anti-inflammatory agent, and important for sensory neuron survival following axotomy. |
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| Catalog # | H1830-51B |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:500-1:1000. |
| Immunohistochemistry (Frozen and paraffin): 1:50. |
| Immunocytochemistry: Frozen and paraffin |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | MCF-7 cells |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5H8 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Prokaryotic recombinant protein corresponding to the full length human hsp27 protein. |
| Specificity | Recognizes HSP27 in human tissues and cell lines at 27 kD. Does not cross react with HSP70, HSP90 or HSP104. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
