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You are here:Home » Antibodies » Antibodies-Heat Shock Proteins » Anti -Heat Shock Protein 27 (HSP27) (BSA & Azide Free)

Anti -Heat Shock Protein 27 (HSP27) (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
HSP27 is identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for HSP27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Catalog #H1830-51X
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlBT474, MDA-MB-453 cells, and breast carcinoma.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No0.N.321
FormSupplied as a liquid in PBS, pH 7.4. No preservatives and no stabilizing proteins added. Also available with BSA and azide. See H1830-51.
PurityPurified by Protein G affinity chromatography.
ImmunogenPartially puri ed HSP27 protein from breast cancer MCF-7 cells. MW
SpecificityRecognizes human HSP27 at 24-27kD. Species Crossreactivity: chimpanzee and monkey. Does not crossreact with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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